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Pyrosequencing Inc locus-specific pyrosequencing
Locus Specific Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/locus-specific+pyrosequencing/locus+specific+pyrosequencing/pmc10022060-325-23-25
Average 90 stars, based on 1 article reviews
locus-specific pyrosequencing - by Bioz Stars, 2026-11
90/100 stars

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Methylation:

Article Title: Depletion of DNMT1 in differentiated human cells highlights key classes of sensitive genes and an interplay with polycomb repression
Article Snippet: We characterised the alterations in methylation and transcription using microarrays in three different cell lines, processing them using a highly reproducible pipeline, and verified changes using locus-specific pyrosequencing or RT-qPCR assays.

Article Title: The effect of high polycyclic aromatic hydrocarbon exposure on biological aging indicators
Article Snippet: Furthermore, we applied the method proposed by Zbieć-Piekarska et al. [ ] to assess DNAmAge, on data from five CpG sites using the locus-specific technology pyrosequencing with some modifications [ , ].

Article Title: DNA Methylation-Based Age Prediction and Telomere Length Reveal an Accelerated Aging in Induced Sputum Cells Compared to Blood Leukocytes: A Pilot Study in COPD Patients
Article Snippet: We applied the method proposed by Zbieć-Piekarska et al. ( ) to assess DNAmAge, on data from five CpG sites using the locus-specific technology pyrosequencing with some modification as described by Pavanello et al. ( , ), which makes the technical analysis achievable in few hours.

Article Title: SLC6A4 methylation modifies the effect of number of traumatic events on risk for posttraumatic stress disorder
Article Snippet: Pyrosequencing validation We used locus-specific pyrosequencing to validate methylation data at at cg22584138.

Quantitative RT-PCR:

Article Title: Depletion of DNMT1 in differentiated human cells highlights key classes of sensitive genes and an interplay with polycomb repression
Article Snippet: We characterised the alterations in methylation and transcription using microarrays in three different cell lines, processing them using a highly reproducible pipeline, and verified changes using locus-specific pyrosequencing or RT-qPCR assays.

Article Title: The effect of high polycyclic aromatic hydrocarbon exposure on biological aging indicators
Article Snippet: Furthermore, we applied the method proposed by Zbieć-Piekarska et al. [ ] to assess DNAmAge, on data from five CpG sites using the locus-specific technology pyrosequencing with some modifications [ , ].

Article Title: DNA Methylation-Based Age Prediction and Telomere Length Reveal an Accelerated Aging in Induced Sputum Cells Compared to Blood Leukocytes: A Pilot Study in COPD Patients
Article Snippet: We applied the method proposed by Zbieć-Piekarska et al. ( ) to assess DNAmAge, on data from five CpG sites using the locus-specific technology pyrosequencing with some modification as described by Pavanello et al. ( , ), which makes the technical analysis achievable in few hours.

Article Title: SLC6A4 methylation modifies the effect of number of traumatic events on risk for posttraumatic stress disorder
Article Snippet: Pyrosequencing validation We used locus-specific pyrosequencing to validate methylation data at at cg22584138.

Biomarker Discovery:

Article Title: Depletion of DNMT1 in differentiated human cells highlights key classes of sensitive genes and an interplay with polycomb repression
Article Snippet: We characterised the alterations in methylation and transcription using microarrays in three different cell lines, processing them using a highly reproducible pipeline, and verified changes using locus-specific pyrosequencing or RT-qPCR assays.

Article Title: The effect of high polycyclic aromatic hydrocarbon exposure on biological aging indicators
Article Snippet: Furthermore, we applied the method proposed by Zbieć-Piekarska et al. [ ] to assess DNAmAge, on data from five CpG sites using the locus-specific technology pyrosequencing with some modifications [ , ].

Article Title: DNA Methylation-Based Age Prediction and Telomere Length Reveal an Accelerated Aging in Induced Sputum Cells Compared to Blood Leukocytes: A Pilot Study in COPD Patients
Article Snippet: We applied the method proposed by Zbieć-Piekarska et al. ( ) to assess DNAmAge, on data from five CpG sites using the locus-specific technology pyrosequencing with some modification as described by Pavanello et al. ( , ), which makes the technical analysis achievable in few hours.

Article Title: SLC6A4 methylation modifies the effect of number of traumatic events on risk for posttraumatic stress disorder
Article Snippet: Pyrosequencing validation We used locus-specific pyrosequencing to validate methylation data at at cg22584138.

Microarray:

Article Title: Depletion of DNMT1 in differentiated human cells highlights key classes of sensitive genes and an interplay with polycomb repression
Article Snippet: We characterised the alterations in methylation and transcription using microarrays in three different cell lines, processing them using a highly reproducible pipeline, and verified changes using locus-specific pyrosequencing or RT-qPCR assays.

Article Title: The effect of high polycyclic aromatic hydrocarbon exposure on biological aging indicators
Article Snippet: Furthermore, we applied the method proposed by Zbieć-Piekarska et al. [ ] to assess DNAmAge, on data from five CpG sites using the locus-specific technology pyrosequencing with some modifications [ , ].

Article Title: DNA Methylation-Based Age Prediction and Telomere Length Reveal an Accelerated Aging in Induced Sputum Cells Compared to Blood Leukocytes: A Pilot Study in COPD Patients
Article Snippet: We applied the method proposed by Zbieć-Piekarska et al. ( ) to assess DNAmAge, on data from five CpG sites using the locus-specific technology pyrosequencing with some modification as described by Pavanello et al. ( , ), which makes the technical analysis achievable in few hours.

Article Title: SLC6A4 methylation modifies the effect of number of traumatic events on risk for posttraumatic stress disorder
Article Snippet: Pyrosequencing validation We used locus-specific pyrosequencing to validate methylation data at at cg22584138.



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